The Institute of Neuropathology has devoted a sizeable amount of work and resources to establishing an automated workflow - aided by a JANUS pipetting robot (PerkinElmer) - which allows to genotype animal biopsies efficiently and with a high throughput. Our efforts have been very successful, and this now enables us to offer our services to other researchers within the USZ.
1 PCR reaction = isolation of genomic DNA from an ear biopsy (transgenic or knockout animals) by specific primers, and analysis on a capillary electrophoresis system.
The PCR results (example), including images of the PCR reaction, are made available online on a password-protected platform.
PCR genotyping service: 7-11CHF/PCR
For each mouse line, a list of the applicable PCR reactions will be defined. For new PCR reactions, clients must supply the primer sequences along with a validated protocol.
www.imice.uzh.ch Online platform for orders and results (registered clients)
>> iMice Registration for new clients
Contact and support:
- PCR results: Karina Arroyo, Karina.Arroyo@usz.ch
- Biopsy handling: Mirzet Delic, Mirzet.Delic@usz.ch
- Software, Database, User-login: Monika Bieri, Monika.Bieri@usz.ch